Coronaviruses : methods and protocols /

This volume aims to describe a variety of techniques that reflects the wide range of research currently performed in the field of coronavirology, and begins with an overview of current understandings of coronavirus replication and pathogenesis to introduce specialists and non-specialists to the fiel... Full description

Other Authors: Maier, Helena Jane,, Bickerton, Erica,, Britton, Paul (Professor),
Format: Book
Language: English
Published: New York : Humana Press : [2015]
Series: Methods in molecular biology (Clifton, N.J.) ; v. 1282.
Springer protocols (Series)
Table of Contents:
  • Coronaviruses : an overview of their replication and pathogenesis /
  • Anthony R. Fehr and Stanley Perlman
  • Identification of a novel coronavirus from Guinea Fowl using metagenomics /
  • Mariette F. Ducatez and Jean-Luc Guérin
  • Serological diagnosis of feline coronavirus infection by immunochromatographic test /
  • Tomomi Takano and Tsutomu Hohdatsu
  • Estimation of evolutionary dynamics and selection pressure in coronaviruses /
  • Muhammad Munir and Martí Cortey
  • Preparation of chicken tracheal organ cultures for virus isolation, propagation, and titration /
  • Ruth M. Hennion
  • Preparation of chicken kidney cell cultures for virus propagation /
  • Ruth M. Hennion and Gillian Hill
  • Isolation and propagation of coronaviruses in embryonated eggs /
  • James S. Guy
  • Characterization of human coronaviruses on well- differentiated human airway epithelial cell cultures /
  • Hulda R. Jonsdottir and Ronald Dijkman
  • Quantification of infectious bronchitis coronavirus by titration in vitro and in ovo /
  • Joeri Kint, Helena Jane Maier, and Erik Jagt
  • Purification of coronavirus virions for Cryo-EM and proteomic analysis /
  • Stuart Dent and Benjamin W. Neuman
  • Partial purification of IBV and subsequent isolation of viral RNA for next-generation sequencing /
  • Sarah M. Keep, Erica Bickerton, and Paul Britton
  • Transient dominant selection for the modification and generation of recombinant infectious bronchitis coronaviruses /
  • Sarah M. Keep, Erica Bickerton, and Paul Britton
  • Engineering infectious cDNAs of coronavirus as bacterial artificial chromosomes /
  • Fernando Almazán [and others]
  • Protein histochemistry using coronaviral spike proteins : studying binding profiles and sialic acid requirements for attachment to tissues /
  • Iresha N. Ambepitiya Wickramasinghe and M. Hélène Verheije
  • Identification of protein receptors for coronaviruses by mass spectrometry /
  • V. Stalin Raj [and others]
  • Single particle tracking assay to study coronavirus membrane fusion /
  • Deirdre A. Costello and Susan Daniel
  • Studying coronavirus-host protein interactions /
  • Chee-Hing Yang [and others]
  • Field-proven yeast two-hybrid protocol used to identify coronavirus-host protein-protein interactions /
  • Pierre- Olivier Vidalain [and others]
  • Investigation of the functional roles of host cell proteins involved in coronavirus infection using highly specific and scalable RNA interference (RNAi) approach /
  • Jean Kaoru Millet and Béatrice Nal
  • Transcriptome analysis of feline infectious peritonitis virus infection /
  • Parvaneh Mehrbod [and others]
  • Quantification of interferon signaling in avian cells /
  • Joeri Kint and Maria Forlenza
  • Studying the dynamics of coronavirus replicative structures /
  • Marne C. Hagemeijer and Cornelis A.M. de Haan
  • Preparation of cultured cells using high-pressure freezing and freeze substitution for subsequent 2D or 3D visualization in the transmission electron microscope / Philippa C. Hawes.